Diazem C18 Columns

Diazem bonding technology takes advantage of both hydrophobic retention mechanisms and secondary acid/base interaction. This yields a linear progressive approach to HPLC separations, and method development becomes a straight forward problem solving tool. Examples of the power of Diazem bonding technology are shown below:

	The Diazem C18-EC column is a standard C18 column. On a 120‰ silica, this column is bonded for high retentivity and is well end-capped to give excellent peak shape. It is a good general purpose column. Column: 150 mm x 4.6 mm Mobile Phase: 50% Phosphate at pH=2.1 / 50% ACN Flow: 1.0 mL/min; Detection: 254 nm; Temp: 30 Deg. C Compounds: 1. 4-Ethylaniline, 2. 3-Ethylaniline, 3. 2-Ethylaniline, 4. Aetophenone

	The Diazem 2000 bonded phase is bonded on a 120‰ silica using proprietary silane pre- and end-cappers to give altered selectivity relative to normal C18 phases. This is a lower retentivity column then the C18 EC. The lower retentivity means quicker run times while still maintaining excellent resolution. Column:: 150 mm x 4.6 mm Mobile Phase: A: 2% H3PO4; 0.005M Pentane Sulfonate B: 50%A / 50%ACN Gradient: 2% B to 30% (10min) Flow: 1.5 mL/min; Detection: 254 nm; Temp: 30 Deg. C Compounds: 1. Metanephrine, 2. 5-hydroxy tryptophan 3. Serotonin, 4. Tryptophan, 5. Methyltryptophan

	The Diazem 1120 is a fast column with excellent chromatographic properties. This C18, on a 120‰ silica, gives new selectivity patterns and is particularly useful for analytes with protonated heteroatoms and some basic sites. Column:: 150 mm x 4.6 mm Mobile Phase: 50% Phosphate at pH=2.1 / 50% ACN Flow: 1.0 mL/min; Detection: 254 nm; Temp: 30 Deg. C Compounds: 1. Carbamazepine, 2. Naproxen 3. Difunisal, 4. Flurbiprofen, 5. Indomethacin

	The Diazem 3000 is truly a unique column chemistry. On a 120‰ silica, this C18 column will give new selectivity patterns. Where every other C18 column fails, this column will solve tough separation problems. Column:: 150 mm x 4.6 mm Mobile Phase: 2% MeOH/H2O; 0.1% TFA Flow: 1.0 mL/min; Detection: 254 nm; Temp: 30 Deg. C Compounds: 1. Uracil, 2. 4-Hydroxybenzoic Acid 3. Methyl-4-hydroxybenzoate, 4. Ethyl-4-hydroxybenzoate, 5. Propyl-4-hydroxybenzoate, 6. Methyl Benzoate

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