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Diazem C8 Columns
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Diazem bonding technology takes advantage of both hydrophobic retention mechanisms and secondary acid/base interaction. This yields a linear progressive approach to HPLC separations, and method development becomes a straight forward problem solving tool. Vitamin SeparationAn example of the power of Diazem bonding technology is shown here on the vitamin separation (see below): |
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| Mobile Phase: A=
7.5 mM Pentanesulfonate / 1% Acetic Acid
B= Methyl Alcohol Gradient: 2% B to 25% B over 25 minutes 150 mm X 4.6 mm columns; 1.5 mL/min; 254 nm |
Compounds:
1. Nicotinamide, 2. p-Aminobenzoic Acid,
3. Pyradoxin (Vitamin B6), 4: Thiamin
(Vitamin B1), 5. Riboflavin (Vitamin B2),
6. Cyanocobalamin (Vitamin B12) |
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Using the same sample and the same gradient conditions, these four C8 Method Development columns give four very different chromatograms with predictable results based on the delta (δ) value of the test columns. The chromatographer does not need to spend hours searching for a method but can simply change the column to change selectivity of an analysis mixture. | ||